Stainless Steel Bioreactor & Fermenter

Stainless Steel Bioreactor Fermenter 10L–500L — Specifications

I. Fermenter Vessel (10L–500L, Customizable Volume)

1.1 Total volume: 10–500 L; filling factor ≥70%.

1.2 Vessel material: 316L stainless steel body, 304 stainless steel jacket with internal baffles.

1.3 Vessel aspect ratio (D:H) ≤ 1:2.5.

1.4 Surface finish: internal surface polished to Ra ≤ 0.4 μm, external brushed finish.

1.5 Pressure design: body/jacket design pressure 0.3/0.4 MPa.

1.6 Top plate ports (minimum): 1 inoculation port; 1 feed inlet; 1 exhaust port; 1 pressure sensor port; 1 antifoam electrode port; 1 stirrer port + 1 spare port; 4 feed ports (4-valve manifold).

1.7 Upper side ports (minimum): 1 gas inlet; 1 rectangular sight glass; 1 rear light glass.

1.8 Lower side ports (minimum): 1 pH electrode port; 1 DO electrode port; 1 temperature electrode port; 2 spare electrode ports; 1 jacket inlet/outlet; 1 sampling port.

II. Stirring System

2.1 Automatic lifting device, AC motor-driven shaft seal, 316L stainless steel agitator shaft.

2.2 Minimum 2 layers of 6-blade flat disc turbines + 1 layer of 4-pitch blade impeller.

2.3 Minimum 1 layer of mechanical foam breaker, height-adjustable, speed range ≥ 50–1000 rpm ±5 (customized per volume), capable of sustained high-speed operation.

III. Temperature Control

3.1 Electric heating with circulating water cooling. Features automatic air venting, water-failure and over-temperature protection. Intelligent PID control for rapid response and energy efficiency.

3.2 Fermentation temperature control range: cooling water temp +5°C to 65°C, accuracy ≤ 0.2°C, resolution ≤ 0.1°C.

IV. Aeration Control

4.1 System includes 2-channel rotameter (air / O₂), 1-stage sterile air filter (0.01 μm). Rotameter + mass flow controller + control valve for automatic flow regulation, range 0–2 VVM.

V. pH Control

5.1 Intelligent PID control with electrode and shielded cable detection. 2 variable-speed peristaltic pumps. pH can be linked with feeding for automatic control and metering.

5.2 Electrode reusable for autoclave sterilization. Calibration fully performed by system software. One spare electrode set included.

5.3 Display range: 0.00–14.00, accuracy ≤ 0.01. Automatic control range: 2.00–12.00, accuracy ≤ 0.05.

VI. Dissolved Oxygen (DO) Control

6.1 Online DO electrode detection. DO can be linked with stirring speed, aeration, feeding and other parameters for cascade control.

6.2 Measurement range: 0–150%, accuracy ≤ 3%, resolution ≤ 0.1%.

6.3 Calibration fully performed by system software.

VII. Foam Control

7.1 Peristaltic pump for automatic addition. Automatic foam detection with variable-speed peristaltic pump and pneumatic diaphragm valve for automatic antifoam dosing. Sensitivity: 100–100,000 Ω.

VIII. Feed Control

8.1 Variable-speed peristaltic pump control. 4 channels of programmable, flip-cover type stepless variable-speed peristaltic pumps.

8.2 Feeding accuracy ≤ 0.02 mL/rev.

8.3 Feeding range: 0.02 mL/min – 340 mL/min.

8.4 Feeding modes: constant-rate, exponential (specific growth rate equation control), linear, sequential, pH-linked, DO-linked, etc.

IX. Exhaust Gas System

9.1 Equipped with exhaust gas condensation system and filter system.

X. Automation System Configuration (Optional)

10.1 Automated sterilization control system: program-controlled, one-touch settings, automatic sterilization. Steam main line equipped with 5 μm pre-filter and pressure reducing valve (range 0–0.4 MPa).

10.2 Automatic air flow detection and control: mass flow meter for inlet air measurement.

10.3 Automatic vessel pressure control: pressure sensor + proportional control valve for exhaust pressure regulation, range 0–0.3 MPa.

XI. RY-HITECH Control System

11.1 Centralized controller with GMP-compliant software for process control and display. Includes on-site 304 SS control cabinet, PLC system, switching power supply, relays, sensors and actuators. HMI uses ≥ 11-inch industrial all-in-one touch panel.

11.2 Control and display parameters include (but not limited to): Temperature, Stirring, pH, DO, Feed, Aeration, Pressure, Broth volume, Cell density, Feed weight, Exhaust O₂ & CO₂, CER, OUR, KLa, RQ.

11.3 Remote management: mobile APP remote control capability.

11.4 Recipe function: fermentation recipe planning and management, ≥ 20 recipe plans.

11.5 Data recording: audit trail, electronic signature, permission management, alarm management. Real-time recording of all parameters and historical trends. Remote control capable, data exportable. Proprietary IP with data recovery and software upgrade services.

11.6 Synchronized parameter setting: synchronized fermentation timing, control settings, parameter settings, pH calibration, DO calibration.

11.7 Parameter comparison curves and comparison reports.

11.8 Real-time video monitoring.

11.9 Control room host computer for real-time remote monitoring.

XII. Control Parameter Modes

12.1 Manual mode: valve ratio or opening percentage settable.

12.2 Automatic mode: PID continuous or PID on/off control selectable.

12.3 Sequential mode: minimum 20 pre-defined control segments for all parameters, enabling automatic phased control. Remote mode: accepts host computer commands.

12.4 Cascade control: DO can be linked with stirring speed, air flow, vessel pressure, feeding, etc.

12.5 pH control via acid and alkali dosing. Metering function for feed, antifoam, acid and alkali.

12.6 Controller capable of controlling ≥ 2 fermenters simultaneously, each operating independently without interference.

12.7 X/Y real-time trend display with flexible zoom-in/out, enabling operators to assess parameter interactions for rapid process adjustments.

12.8 Data processing: trend curves for all parameters. Historical data storage, display and analysis.

12.9 Batch reports for all parameters. Direct USB data export.

12.10 Online curve screen capture. Data export in PDF and Excel formats.

12.11 Access control management: three-tier permissions (OPE/MON/SYS), each tier supports multiple sub-accounts.